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Tuesday, April 28, 2020 | History

2 edition of Glutamine synthesis in Rhizobium meliloti found in the catalog.

Glutamine synthesis in Rhizobium meliloti

Robert G. Shatters

Glutamine synthesis in Rhizobium meliloti

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Published .
Written in English

    Subjects:
  • Rhizobium meliloti.

  • Edition Notes

    Statementby Robert G. Shatters, Jr.
    The Physical Object
    Paginationxii, 159 leaves, bound :
    Number of Pages159
    ID Numbers
    Open LibraryOL16762100M

      Genetic Mapping of Rhizobium meliloti using RP Nitrogen Fixation (NIF) Regulatory Mutants of Klebsiella: Determination of the Energy Cost of N2 Fixation in vivo.- Cloning Nitrogen Fixing Genes from Klebsiella pneumoniae in vitro and the Isolation of NIF Promoter Mutants Affecting Glutamine Synthetase Regulation.-Author: Alexander Hollaender.   Root exudates of alfalfa (Medicago sativa L.) inoculated with symbiotic Rhizobium meliloti bacteria contained three isoflavonoids that were not found in exudates of uninoculated plants. Data from proton nuclear magnetic resonance, mass spectrometry, and ultraviolet-visible absorbance analyses indicated that root exudates of inoculated plants contained aglycone and glycoside forms of Cited by:


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Glutamine synthesis in Rhizobium meliloti by Robert G. Shatters Download PDF EPUB FB2

We have determined the DNA sequence of aRhizobium meliloti gene that encodes glutamine synthetase II (GSII). The deduced amino acid Glutamine synthesis in Rhizobium meliloti book was compared to that of Bradyrhizobium japonicum GSII and those of various plant and mammalian glutamine synthetases (GS) in order to evaluate a proposal that the gene for this enzyme was recently transferred from plants to Cited by: Sinorhizobium meliloti and Rhizobium etli CE3 turn over nitrogen and carbon from glutamine to ammonium and CO2, respectively.

Some of the ammonium released is assimilated back into glutamine. weextendthis analysis toRhizobium melilotiandshowthat R. meliloti contains three Glutamine synthesis in Rhizobium meliloti book loci involved in glutamine synthesis (ginA, glnlI, and glnT), which are differentially regulated in response to fluctuating ammonium concentra-tions and which appear to be nonessential for Glutamine synthesis in Rhizobium meliloti book nitrogen fixation.

(Preliminary aspects ofthis workhavebeendescribed byCited by: Regulation of glutamine synthetase II activity in Rhizobium meliloti A14 Article (PDF Available) in Journal of Bacteriology (9) October with 24 Reads How we measure 'reads'. Abstract Glutamine synthetase I from Rhizobium meliloti was found to be inhibited by adenosine 5′-monophosphate, alanine, glycine, carbamyl phosphate, cytidine 5′-triphosphate, tryptophan, histidine, and glucosaminephosphate.

Each inhibitor was independent in its action and the effect was cumulative when more than one inhibitor was by: 1. meliloti ntrA (35) and ntrC (39) mutants have been Glutamine synthesis in Rhizobium meliloti book, but neither mutantis aglutamine auxotroph. To understand the role GSII might play in rhizobial nitrogen metabolism, wefoundmutantsthat wereunable to express GSII, by isolating glutamine auxotrophs derived from an R.

meliloti glnA mutant (38a). Wereport here thatCited by:   Rhizobium meliloti, like many other bacteria, accumulates high levels of glutamic acid when osmotically stressed.

The effect was found to be proportional to the osmolarity of the growth medium. NaCl, KCI, sucrose, and polyethylene glycol elicited this response.

The intracellular levels of glutamate and K+ began to increase immediately when cells were shifted to high-osmolarity by: Osmoregulation in Rhizobium meliloti: Production ofGlutamic Acid in Response to Osmotic Stress JAMESL.

BOTSFORD*ANDTHOMASA. LEWIS DepartmentofBiology, NewMexicoState University, Las Cruces, NewMexico Received 28 July /Accepted 17 November Rhizobium meliloti, like many other bacteria, accumulates high levels of glutamic acid when Cited by: diminished metabolic flow through the rhizobial pathways for the synthesis of purines and pyrimidines and the synthesis of biotin, nicotinic acid, riboflavin and thiamine by rhizobium appear to be requirements for normal symbiosis.

biosynthetic pathways of 17 amino acids in symbiosis Glutamine Three di stinct S. meliloti loci involved Cited by:   Watson, R. & Rastogi, V. Cloning and nucleotide sequencing of Glutamine synthesis in Rhizobium meliloti book meliloti aminotransferase genes: an aspartate aminotransferase required.

Rhizobium meliloti, like many bacteria, accumulates elevated levels of glutamate when osmotically biochemical basis for this increase in glutamate production was investigated. Enzymes involved in glutamate synthesis, including glutamine synthetase, glutamate synthase, and glutamic dehydrogenase, were characterized in dialyzed crude cell-free by: Because glutamine synthetase (GS) is the key step of ammonium assimilation in Rhizobium, we studied the regulation of GS expression or activity and its role during symbiosis.

The alfalfa symbiont Rhizobium meliloti contains three different GSs, termed GSI, GSII Glutamine synthesis in Rhizobium meliloti book GSIII. GSII is not expressed in Cited by:   Somerville JE, Kahn ML. Cloning of the glutamine synthetase I gene from Rhizobium meliloti. J Bacteriol.

Oct; (1)– [PMC free article] Tempest DW, Meers JL, Brown CM. Synthesis of glutamate in Aerobacter aerogenes by a hitherto. Journals & Books; Help; COVID campus closures: Symbiotic nitrogen fixation does not require adenylylation of glutamine synthetase I in Rhizobium meliloti.

Author links open overlay panel Tania Arcondéguy Isabelle Huez 1 Joëlle Fourment Daniel Kahn Cited by: Total synthesis of the sulfated lipooligosaccharide signal involved in rhizobium meliloti-alfalfa symbiosis. Tetrahedron Letters34 (48), DOI: /S(00) Hideki Maeta, Takashi Matsumoto, Keisuke Suzuki.

Most rhizobia contain two glutamine synthetase (GS) enzymes: GSI, encoded by glnA, and GSII, encoded by glnII. We have found that WSU, a Rhizobium meliloti A14 glutamine auxotroph derived from a glnA parental strain, is an ntrA by: A total synthesis of the sulfated lipotetrasaccharide (NodRm-1) is described.

First, the disaccharide glycosyl donor—O-(2-azido-3,4,6-tri-O-benzyldeoxy-β-D-glucopyranosyl)-(1 →4)-3,6-di-O-benzyldeoxyphthalimido-β-D-glucopyranosyl chloride 3a or the corresponding trichloroacetimidate 3b— and the disaccharide glycosyl acceptor — benzyl O-(3,6-di-O-benzyldeoxyphthalimido.

Two forms of glutamine synthetase are found in members of the genus Rhizobium, a heat-stable glutamine synthetase I (GSI) and a heat-labile GSII. As a step toward clarifying the role of these enzymes in symbiotic nitrogen fixation, we have cloned the structural gene for GSI from Rhizobium meliloti A Rhizobium meliloti and Rhizobium leguminosarum have a third GS (GSIII) encoded by glnT (Espin et al, ; Shatters et al, ).

Pseudanabaena sp. strain PCC is a cyanobacterium that lacks the typical prokaryotic glutamine synthetase type I encoded by the glnA gene (Crespo et al, ).

Key words: N-acetylglutaminylglutamine amide, osmoregulation, Rhizobium meliloti Abstract Rhizobium meliloti adapts to environments of high osmolarity by accumulating glutamate, trehalose, and the dipeptide N-acetylglutaminylglutamine amide (NAGGN) intracellularly.

In this study, the mechanism of NAGGN production and accumulation was examined. The stereocontrolled synthesis of the sodium salt of NodRm IV (S), the bacterial chemical signal involved in the Rhizobium meliloti-alfalfa symbiosis, has been achieved in an overall yield of 8%.

You have access to this articleCited by:   In various Rhizobium species, such as R. leguminosarum and R. meliloti, common and host-specific nodullation (nod) genes have been identified which determine infection and Cited by: The pathways of ammonium assimilation in Rhizobium meliloti Ali, Hassan; Niel, Christian; Guillaume, Jean Two pathways of ammonium assimilation are known in bacteria, one mediated by glutamate dehydrogenase, the other by glutamine synthetase and glutamate synthase.

The activities of these three enzymes were measured in crude. Abstract. A symbiotically important gene system in rhizobial species is the heme biosynthetic pathway. A mutant having reduced levels of delta-aminolevulinic acid synthetase, the first unique enzyme in this pathway, was obtained in Rhizobium meliloti F34 by N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis.

/ Six nodulation genes of nod box locus 4 in Rhizobium meliloti are involved in nodulation signal production: nodM codes for d-glucosamine synthetase. In: MGG Molecular & General Genetics. ; Vol. No. Cited by:   Genetics Of Legume Rhizobium Symbiosis [involving glutamine synthetase (GS)[and an NADPH-dependent glutamineoxoglutarate amidotransferase (GOGAT), must be operating when ammonia is present in the growth medium at low levels.

and sometimes elicit roothair deformation reactions on plants that arenotusually hosts. loss of nodH function. Abstract. Bami cultivar of alfalfa (Medicago sativa) was inoculated with salt-tolerant Sinorhizobium meliloti in solution culture with different salt concentrations (0, 50, 75 and mmoles NaCl) added immediately at the time of inoculation.

The results indicated that S. meliloti formed an infective and effective symbiosis with alfalfa under saline and nonsaline conditions. To inhibit expression specifically in the phloem, a bp fragment of a cDNA (Gln) encoding cytosolic glutamine synthetase (GS1) from tobacco was placed in the antisense orientation downstream of the cytosolic Cu/Zn superoxide dismutase promoter of Nicotiana plumbaginifolia.

After Agrobacterium-mediated transformation, two transgenic N. tabacum lines exhibiting reduced levels of Cited by:   Abstract. Using whole genome arrays, we systematically investigated nitrogen regulation in the plant symbiotic bacterium Sinorhizobium use of glutamate instead of ammonium as a nitrogen source induced nitrogen catabolic genes independently of the carbon source, including two glutamine synthetase genes, various aminoacid transporters and the glnKamtB by: title = "Glutamine utilization by Rhizobium etli", abstract = "We undertook the study of the use of glutamine (Gln) as the source of carbon and energy by Rhizobium etli.

Tn5-induced mutagenesis allowed us to identify several genes required for Gln utilization, including those coding for two broad-range amino acid transporters and a glutamate Cited by: The isolation and characterization of a Rhizobium etli glutamate auxotroph, TAD12, harbouring a single Tn5 insertion, is reported.

This mutant produced no detectable glutamate synthase (GOGAT) activity. The cloning and physical characterization of a 72 kb fragment of R.

etli DNA harbouring the structural genes gltB and gltD encoding the two GOGAT subunits GltB and GltD is also by: Overview of nitrogen fixation; Plasmids as vectors for gene cloning; The nitrogen fixation cistrons of Klebsiella pneumoniae; Transformation of nitrogen-fixation genes in Azotobacter; Genetic hybridization of root-nodule bacteria (Rhizobium); Genetic mapping of Rhizobium meliloti using RP4; Nitrogen fixation (NIF) regulatory mutants of Klebsiella: determination of the energy cost of N2.

Proteomes - Rhizobium meliloti (strain ) (Ensifer meliloti) (Sinorhizobium meliloti)))) All None. Overview "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain " Capela D., Barloy-Hubler F., Gouzy J., Bothe G.

To analyze the overexpression of the Rhizobium meliloti fdxN gene in Escherichia coli, different translational and transcriptional fusions were translational signals of ti fdxN were recognized in as demonstrated by the use of in-frame lac fusions. Translational fusions consisting of the lacZ or the lpp gene fused in frame to the 3′ end of the entire fdxN gene Cited by: 1.

Nodulation (nod) genes are required for Rhizobium meliloti to invade and stimulate nodule formation in its host, have established the DNA sequence of nodD, nodA, and nodB, which are part of a gene cluster located 20 kb downstream of nifHDK on the R. meliloti pSym megaplasmid.

The nodD open reading frame ( amino acids) reads from proximal to nifHDK toward distal to nifHDK. Rhizobium meliloti (strain ) (Ensifer meliloti) (Sinorhizobium meliloti) Status. Hydrogenobyrinate a,c-diamide synthase (cobB) The N-terminal domain is anticipated to bind ATP and hydrogenobyrinate and catalyzes the ultimate synthesis of the diamide product.

The ammonia produced via the glutaminase domain is probably translocated to. Sinorhizobium meliloti is a Gram-negative bacterium which fixes atmospheric nitrogen. It forms a symbiotic relationship with legumes from the genera Medicago, Melilotus and Trigonella, including the model legume Medicago symbiosis results in a new plant organ termed a root nodule and is deemed symbiotic as it leaves excess nitrogen behind for the : Alpha Proteobacteria.

Glutamine Synthesis in Rhizobium meliloti. Ph.D. Thesis, Washington State University. Progress 01/01/87 to 12/30/87 Outputs Glutamate catabolism in Rhizobium meliloti has been studied by assaying the activity of various catabolic enzymes, studying the fate of radioactive glutamate in extracts and in whole cells and by isolating mutants unable.

Wiley Online Library will be unavailable on Saturday 14th May BST / EDT / SGT for essential ies for the inconvenience. Rhizobium is a genus of Gram-negative soil bacteria that fix nitrogen.

Rhizobium species form an endosymbiotic nitrogen-fixing association with roots of legumes and Parasponia. The bacteria colonize plant cells within root nodules, where they convert atmospheric nitrogen into ammonia using the enzyme nitrogenase and then provide organic nitrogenous compounds such as glutamine or ureides to Class: Alphaproteobacteria.

Sinorhizobium meliloti Rm pdf to sudden shifts to nitrogen pdf carbon starvation conditions by an accumulation of the stringent response alarmone ppGpp and remodelling of the transcriptome.

The gene product of relA, Rel Sm, responsible for synthesis of ppGpp, shows functional similarities to E. coli SpoT. Using promoter–egfp gene fusions, we showed that in Rm relA is expressed at Cited by:   Sinorhizobium meliloti nodulation factors (NFs) elicit a number of symbiotic responses download pdf alfalfa (Medicago sativa) roots.

Using a semiquantitative nodulation assay, we have shown that chemically synthesized NFs trigger nodule formation in the same range of concentrations (down to 10−10 m) as natural NFs.

The absence of O - sulfate or O- acetate substitutions resulted in a decrease in.However, glutamine synthesis seems to ebook important at several stages in the symbiosis, ebook a B. japonicum glnA,glnII double mutant is a glutamine auxotroph and is defective in nodulation (Carlson et al.

), whereas in R meliloti a glnA,glnII mutant, which under certain conditions is not a glutamine auxotroph, has a Nod + Fix.